Composite
Part:BBa_K2036018:Design
Designed by: Zhangyu Cheng Group: iGEM16_HUST-China (2016-09-20)
pRE-RBS-Cro-RBS-CII-TT-ptrp2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is Cro and CII tandom expression. CII expressed at the downtream of pRE can form a positive feedback to enhance Cro's expression to reach a ideal exppression level.
Source
pRE, Cro and CII are all from bacteriaphage lambda.