Composite

Part:BBa_K2036018:Design

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-20)


pRE-RBS-Cro-RBS-CII-TT-ptrp2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is Cro and CII tandom expression. CII expressed at the downtream of pRE can form a positive feedback to enhance Cro's expression to reach a ideal exppression level.


Source

pRE, Cro and CII are all from bacteriaphage lambda.

References